Yazar "Altun, Hatice Uludağ" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Comparison of manual and automated nucleic acid isolation methods for HBV-DNA and HCV-RNA assays
    (EDIMES Edizioni Medico Scientifiche, 2015) Yağmur, Gülhan; Altun, Hatice Uludağ; Gökahmetòğlu, Selma D.; Basok, Ela
    In the diagnosis and monitoring of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, it is important to use methods that can provide rapid and reliable results. The present study aimed to compare the automated and manual extraction methods during the nucleic acid isolation phase for HBV-DNA and HCVRNA assays. The study included 93 serum samples, 49 of which were for the HBV-DNA assay and 44 for the HCVRNA assay. DNA and RNA isolation from the samples was performed manually with a “QIAmpMin Elute Kit” (Qiagen, Germany) and the automated isolation system, NucliSens easyMAG (BioMérieux, France). All the extraction products were amplified using the iCycler device (Bio-Rad, USA). With both methods, compliance was found in 21 (42.8%) samples in the HBV-DNA assay; nine (18.3%) samples had a higher amount of viral nucleic acid with the manual method, whereas 19 samples (38.7%) were found to have a higher amount of nucleic acid with the automated system. For the HCV-RNA assay, total compliance was found in 31 (70.4%) samples; 12 (27.2%) samples had a higher amount of viral nucleic acid with the manual method whereas one sample (2.2%) was found to have a higher amount of nucleic acid with the automated system. It was concluded that the NucliSens easy- MAG automated isolation system can be used with confidence for nucleic acid extraction due to its higher sensitivity, providing results in a shorter time, and assured standardization. © 2021 Elsevier B.V., All rights reserved.
  • Küçük Resim Yok
    Öğe
    The specificity and sensitivity results of the rapid antigen test used in the diagnosis of group A beta hemolytic streptococcal tonsillopharyngitis
    (Acta Medica Mediterranea, 2015) Altun, Hatice Uludağ; Meral, Tuba; Arıbaş, Emel Türk
    Aims: The rapid antigen detection test and throat culture can be used for the diagnosis of group A beta hemolytic streptococcus (GABHS) infections. The aim of this study was to determine the sensitivity and specificity ratios of the rapid antigen test for GABHS in the laboratory setting. Materials and methods: In this study, the throat culture and rapid antigen test results were evaluated for 5120 patients between the ages of 0-18 years, who were admitted between January and June 2014 to the pediatric outpatient clinic with a diagnosis of clinical exudative tonsillopharyngitis. The tests of these patients were performed at the microbiology laboratory of Turgut Ozal University Hospital. Patients with only a throat culture or a rapid antigen test result were excluded from the study. Thus, 1243 patients were included in the current study, in which both tests had been performed. Two throat swab samples were collected from these patients. Culture tests and rapid antigen tests were both performed for the patient samples. The Strep A Abon kit [Hangzhou, China] was used as the rapid antigen test. Results: Nine hundred thirty-six patients had no bacterial growth in their throat cultures, while 307 throat cultures were positive for GABHS. The sensitivity and specificity of the rapid antigen test were 73% and 96.8%, respectively. The positive and negative predictive values for the rapid antigen test were 88.2% and 91.6%, respectively. Conclusion: Although the specificity of the rapid antigen test used in this study was high (96.8%), its sensitivity was determined to be lower (73%). Therefore, for patients in whom negative test results are obtained, it would be appropriate to confirm the test results with throat cultures. © 2020 Elsevier B.V., All rights reserved.