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    Effects of iloprost infusion on the eyes due to ovarian ischemia reperfusion ınjury: an experimental study in rats
    (Malatya Turgut Özal Üniversitesi, 2022) Tosun, Fadime; Annaç, Ebru; Doğukan, Mevlüt; Duran, Mehmet; Uludag, Öznur
    Background: In this study, it is aimed to investigate the effect of iloprost treatment on the eye, which is a prostaglandin analogue, given to rats after the ovarian ischemia-reperfusion model and to contribute to the literature.Materials and Methods: A total of 32 female Sprague Dawley rats were used for the experiment. Rats were divided into four groups of eight rat search: control group, ischemia group (I), ischemia-reperfusion (I-R) group, I-R/I. Except for the control group, torsion procedure was performed on all rats. 3-hour torsion in the ischemia group, 3-hour torsion and 3-hour detorsion in the I-R group, 3 hour torsion, 3 hour detorsion and 60 mn intravenous iloprost infusion in the I-R/I. After the procedures in each group, the eye tissues were taken for histopathological examination.Results: In the histopathological examination of the corneal layer, many large gaps and (decreased collagen cross-linking) were detected between the stromal collagen fiber bundles in group I, I-R and I-R/I unlike the control group. In the histopathological examination of the retinal layer and stroma of the iris, nohemoragicarea and inflammation findings were observed in the examinations performed in all groups, but vascular dilatation was found in groups I, I-R and I-R/I when compared to the control group. A higher increase in vascular dilatation was detected in group I-R/I compared to the other groups.Conclusions: After I/R, although histopathological changes in the eye tissue such as morphological disorders in the stromal collagen fiber bundles of the corneal layer and vascular dilatation in the stroma of iris are supported by the literature, the protective effect of iloprost on tissue damage has not been fully determined.
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    Histopathological investigation of the effects of trastuzumab on the uterus in a rat model with endometriosis
    (2025) Kırıcı, Pınar; Annaç, Ebru; Deniz, Ömür Gülsüm; Kaplan, Selçuk
    It was aimed to investigate histopathological effects of trastuzumab on the uterus in a rat model with experimentally induced endometriotic tissue in the present study. In this study, 28 female Wistar albino rats (10-12 weeks old, 250-280 g) were divided into 4 groups (n =7). After a 7-day acclimation period, rats in the estrous phase were selected. The control group received no endometriosis induction; fat tissue was attached to the peritoneum and the abdomen was sutured. The endometriosis group underwent surgical induction of endometriosis using an auto-transplantation method, where uterine horn fragments were sutured to the peritoneum and mesentery. After a 4-week recovery period, the lesion size was measured, but no treatment was given. The endometriosis+Trastuzumab group received the same induction procedure as the endometriosis group, followed by intraperitoneal administration of trastuzumab (5 mg/kg) for 4 weeks. The trastuzumab group had no endometriosis induction but received the same trastuzumab dosage and schedule. Every animal tissue sample was obtained, and histopathological analysis was performed. In histopathological analysis, there was a significant difference between the Endometriosis group which had the most severe pathological changes compared to the control group (p<0.01). As well as; Endometriosis+Trastuzumab groups showed significant improvement in terms of epithelial integrity (p<0.05), Mast cell infiltration (p<0.01), glandular degeneration (p<0.05), and fibrosis (p<0.05), compared with the endometriosis group. In addition, no statistically significant difference was detected in the control and trastuzumab groups (p>0.05). Trastuzumab treatment resulted in protective effects on endometriosis-affected uterine tissue. In this context, histopathological results suggested that trastuzumab may be beneficial in treating damage caused by experimentally induced endometriosis.
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    Protective Effect of Pomegranate Juice on Lead Acetate-Induced Liver Toxicity in Male Rats
    (2024) PEKMEZ, HIDIR; Annaç, Ebru; Bulmus, Ozgur; Zencirci, Büşra; aydın, merve; Aydın, Ali
    Objective: Lead has been reported to cause oxidative stress in liver tissues and cause histopathological changes. Studies have shown that pomegranate juice has antioxidant properties that prevent oxidative stress. In this study, the harmful effects of lead acetate on rat liver tissue and the efficacy of pomegranate juice against these effects were investigated. Methods: 28 male Wistar albino rats were divided into four groups: control, lead acetate (50 mL/ kg), pomegranate juice (1 mL/kg), and lead acetate + pomegranate juice (50 mL/kg+1 mL/kg). Lead acetate and pomegranate juice were administered orally. Results: When compared with the control group, it was seen that the lead acetate had an increase in the malondialdehyde level and a decrease in reduced Glutathione, Glutathione S-transferase, and Carboxylesterases. Group lead acetate + pomegranate juice had a reduction in malondialdehyde level and an increase in Glutathione, Glutathione S-transferase, and Carboxylesterases compared with the group lead acetate. The lead level of group lead acetate + pomegranate juice decreased compared to the group lead acetate. Cellular degeneration and irregular hepatic cords were observed in group lead acetate’s liver tissue, and the negative changes were lost in group lead acetate + pomegranate juice. Conclusion: It was observed that pomegranate juice had a protective effect against liver toxicity caused by lead acetate.
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    The protective effects of pomegranate juice on lead acetate-induced neurotoxicity in the male rat: A histomorphometric and biochemical study
    (Wiley, 2021) Annaç, Ebru; Uçkun, Miraç; Özkaya, Ahmet; Yoloğlu, Ertan; Pekmez, Hıdır; Bulmuş, Özgür; Aydın, Ali
    The purpose of this study was to investigate the potential side-effects of lead acetate (LA), which is toxic to the nerves, blood and muscles, in the rat brain. The neuroprotective effects of pomegranate juice (PJ) against LA exposure were also observed. The experiment involved 28 male Wistar albino rats aged 12 weeks. These were divided into four groups: Control, PJ, LA and LA+PJ. Stereological techniques were employed to determine hippocampal volume in each rat brain. Biochemical investigations and histopathological examinations were also performed. Analysis demonstrated a significant decrease in hippocampal volume in the LA group compared to the control group (p < .05). The stereology results also indicated that PJ has protective effects when compared with the LA and LA+PJ groups. A significant increase was also determined in malondialdehyde (MDA) levels and glutathione S-transferase (GST) activity in the LA group compared to the control group, in contrast to glutathione (GSH) levels and carboxylesterase (CaE) and acetylcholinesterase (AchE) activities. MDA and GST activity decreased significantly in the LA+PJ group compared to the LA group in contrast to GSH levels and CaE and AchE activities. Histopathological examination revealed a number of degenerative changes in the LA group. Exposure to LA adversely affects the hippocampus on the male rat brain. It might also be suggested that PJ may ameliorate these deleterious effects.
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    Ratlarda siklofosfamid kaynaklı kardiyotoksisite üzerine timokinonun etkileri
    (Malatya Turgut Ozal University, 2023) Yanılmaz, Elif Merve Betül; Memi, Gülsün; Annaç, Ebru; Kaya, Mehmet Salih
    Amaç: Antineoplastik kemoterapide kullanılan bir ajan olan siklofosfamidin (CP) birçok doku üzerinde toksik etkisi bulunmaktadır. Bu çalışmada siklofosfamidin oluşturduğu kardiyotoksisiteye karşı çörek otu uçucu yağının baskın bileşeni olan Timokinonun (TQ) koruyucu etkileri araştırıldı. Materyal ve Metot: Bu amaçla 32 adet 8 haftalık erkek Sprague Dawley sıçan 4 eşit gruba ayrıldı (n=8). Kontrol, TQ (10 mg/kg/gün, oral gavaj), CP (50 mg/kg, i.p., 1, 8, 15 ve 22. günlerde) ve TQ+CP (TQ 10 mg/kg/gün, oral gavaj ve CP 50 mg/kg, i.p., 1, 8, 15 ve 22. günlerde). Dört haftalık uygulamaların ardından kalp dokusunda histopatolojik ve biyokimyasal analizler yapıldı. Bulgular: Kalp dokusunda CP’nin malondialdehit (MDA) seviyelerinde belirgin artışlara sebep olduğu (p
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    The Effects of Nateglinide and Octreotide on the Uterus in Rats with Experimentally Developed Polycystic Ovary Syndrome: A Histopathological Study
    (2026) Kırıcı, Pınar; Annaç, Ebru; Deniz, Ömür Gülsüm; Kaplan, Selçuk
    Objectives: It was aimed to investigate the histopathological effects of Nateglinide (NG) and Octreotide (OC) on uterine morphology in rats with experimentally induced polycystic ovary syndrome (PCOS). Methods: Forty-two female Sprague-Dawley rats (10-12 weeks old, 340-360 g) were divided into six groups (n=7 per group) as Control, PCOS, PCOS+NG, NG only, PCOS+OC, OC only. PCOS was induced via daily oral administration of Letrozole (1 mg/kg) for 21 days. Treatment groups received NG (oral, 30 days) or OC (intraperitoneal, 0.1 mg/kg/day for 30 days). After the experiment, the uterus tissues of all rats were dissected and subjected to histopathological examinations after histological procedures. Results: Histopathological analysis revealed significant uterine damage in the PCOS group compared to other groups (P<0.01). In contrast, the Control, NG-only, and OC-only groups showed normal uterine architecture with intact epithelium, organized glands, and normal stromal structure and there were no significant differences between related groups (P>0.05). Treatment with NG or OC in PCOS rats led to improved epithelial and glandular morphology and reduced Mast cell density, no evidence of edema, and inflammation was found in the connective tissue of these treated groups, suggesting partial improvement of PCOS-induced uterine pathology (P<0.01). Conclusions: NG and OC treatments ameliorated PCOS-induced uterine histopathological changes, suggesting their potential to improve endometrial morphology. These findings may have implications for therapeutic strategies aimed at enhancing endometrial receptivity and highlighting the importance of addressing endometrial health in therapeutic strategies beyond ovarian treatment in PCOS patients.