Yazar "Ceylan, Furkan Sacit" seçeneğine göre listele

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    Investigation of the effects of 5-fluorouracil and thymoquinone on head and neck cancer stem cells
    (Canadian Soc Clinical Investigation, 2015) Acar, Muradiye; Gunduz, Mehmet; Ocak, Tarik; Gurses, Hacer Esra; Cetin, Elif Nihan; Ceylan, Furkan Sacit; Sefa, Alperen
    Purpose: A subpopulation of cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC) play a critical role in invasion, metastasis, and tumour recurrence post-therapy. 5-Fluorouracil (5-FU) is a pyrimidine analogue that is used in the treatment of HNSCC. Thymoquinone (TQ), found in Cumin (Nigella sativa) seed oil, has anti-diabetic, anti-oxidant, anti-inflammatory, and antitumour effects. The purpose of this study was to examine the effects of 5-FU and TQ on CSCs. Methods: ALDH1 (+) cells were isolated from the HNSCC cell line UT-SCC-74A using magnetic activated cell sorting (MACS) and sphere formation was confirmed. Real-time RTPCR revealed was used to measure expression levels of a number of stem cell markers, including OCT4, SOX2, and KLF-4. To investigate the effects of the therapeutic agents, 5-FU and TQ, concentration-dependent assay procedures were used and cell viability after treatment was measured using XTT assay. Results: ALDH1 (+) cells formed spheres while the ALDH1 (-) cells did not. Real-time RT-PCR revealed that a number of stem cell markers, including OCT4, SOX2, and KLF-4, were up-regulated in the CSCs. XTT results showed that, when given alone at a dose of 50 ng/mL, neither agent had a statistically significant cytotoxic effect on CSCs. However, when dosed in combination, TQ and 5-FU had a synergistic effect on CSC cytotoxicity. Conclusion: We report the effects of TQ and 5-FU on CSCs in HNSCC.
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    Proceedings of peer-reviewed and selected articles from Turgut Ozal University Faculty of Medicine 7th International Student Congress
    (The Canadian Society for Clinical Investigation, 2016) Ceylan, Furkan Sacit
    Turgut Ozal University Scientifc Research Committee (TOBAT) was established at Turgut Ozal University Faculty of Medicine in 2009 to encourage young medical students and scientists to carry out novel scientifc research in addition to their medical education. Every year a committee (Chair, Student Member and Scientifc and Social Committees, and Advisory Chair) is set up by the volunteer students and their advisors to organize the congress, with the help of the previous year's Committee © 2021 Elsevier B.V., All rights reserved.
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    Understanding Thermal Sensitivity At The Molecular Level And Developing Temperature-Based Systems Using Rna Termometers
    (The Canadian Society for Clinical Investigation, 2016) Gul, Fatih; Orhan, Ibrahim Yasir; Ceylan, Furkan Sacit; Akdeniz, Nadir B.; Karadag, Hizir Abdulkadir
    Purpose: Temperature sensitivity is found in all multicelleular organisms, as well as in most primitive life forms. Te ubiquity of this temperature sensitivity is an indicator of its efects at the multicellular, cellular and molecular levels [1]. Previous studies have shown that temperature-based regulation is present in the transcriptional process [2]. RNA Termometers, temperature-sensitive sequences, have been shown to act on heat-shock genes to regulate temperature-dependant systems in many organisms [3,4]. Te goal of this study was to characterize the shifs in the functioning of these RNA Termometers at various temperatures. In addition, using the principle of transcriptional thermoregulation, an automated temperature-responsive system stimulating inverse endothermic and exothermic enzymatic reactions for heat stabilization was proposed. Methods: Te endothermic enzymatic reaction was designated as the breakdown of urea, refecting the function of urease, and the exothermic reaction was designated as the breakdown of hydrogen peroxide, refecting the function of catalase [5]. Results: Te proposed system was built upon the translation of urease and the inhibition of catalase translation at higher temperatures, and the inverse at lower temperatures. As RNA Termometers can be used only to drive transcription at higher temperatures, the installation of a lac-regulated 2-way system was suggested. Tis system would also provide a synthetic solution to thermoregulation and the current systems employed today. Tis system could be applied where the current thermoregulatory systems prove insufcient and could be further developed and optimized to replace them in the future © 2021 Elsevier B.V., All rights reserved.