Yazar "Gunduz, Ayten" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim Yok
    Öğe
    Ameliorative Effects of Larazotide Acetate on Intestinal Permeability and Bacterial Translocation in Acute Pancreatitis Model in Rats
    (Springer, 2024) Karahan, Dogu; Harputluoglu, Muhsin Murat Muhip; Gul, Mehmet; Gunduz, Ayten; Ozyalin, Fatma; Inceoglu, Feyza; Satilmis, Basri
    Background Intestinal barrier dysfunction in acute pancreatitis (AP) may progress to systemic inflammatory response syndrome (SIRS) and multi-organ failures by causing bacterial translocation. Larazotide acetate (LA) is a molecule that acts as a tight junction (TJ) regulator by blocking zonulin (Zo) receptors in the intestine. Aims In our study, we aimed to investigate the effects of LA on intestinal barrier dysfunction and bacterial translocation in the AP model in rats. Methods Thirty-two male Sprague-Dawley rats were divided into 4 groups; control, larazotide (LAR), AP, and AP + LAR. The AP model was created by administering 250 mg/100 g bm L-Arginine intraperitoneally 2 times with an hour interval. AP + LAR group received prophylactic 0.01 mg/mL LA orally for 7 days before the first dose of L-Arginine. For intestinal permeability analysis, fluorescein isothiocyanate-dextran (FITC-Dextran) was applied to rats by gavage. The positivity of any of the liver, small intestine mesentery, and spleen cultures were defined as bacterial translocation. Histopathologically damage and zonulin immunoreactivity in the intestine were investigated. Results Compared to the control group, the intestinal damage scores, anti-Zo-1 immunoreactivity H-Score, serum FITC-Dextran levels and bacterial translocation frequency (100% versus 0%) in the AP group were significantly higher (all p < 0.01). Intestinal damage scores, anti-Zo-1 immunoreactivity H-score, serum FITC-Dextran levels, and bacterial translocation frequency (50% versus 100%) were significantly lower in the AP + LAR group compared to the AP group (all p < 0.01). Conclusions Our findings show that LA reduces the increased intestinal permeability and intestinal damage by its effect on Zo in the AP model in rats, and decreases the frequency of bacterial translocation as a result of these positive effects.
  • Küçük Resim Yok
    Öğe
    ANTIBIOTIC SENSITIVITY OF Escherichia Coli STRAINS ISOLATED FROM DIFFERENT SAMPLES: 5-YEAR RETROSPECTIVE ANALYSIS
    (Nobel Ilac, 2023) Gunduz, Ayten; Mansur, Ahmet
    Objective: This study, it was aimed to investigate the antibiotic susceptibility of Escherichia coli (E. coli) isolates isolated from different samples sent to the microbiology laboratory of our hospital in five years retrospectively.Material and Method: 3422 E. coli strains isolated from different samples sent to Malatya Turgut ozal University Faculty of Medicine Malatya Training and Research Hospital Microbiology Laboratory between January 2015 and December 2019 was evaluated retrospectively within the scope of this study. Identification and antimicrobial susceptibility tests (ADT) of all isolates were performed with the VITEK 2 & REG; automated system. Antimicrobial susceptibility tests and interpretation of results were made in accordance with the recommendations of The European Committee on Antimicrobial Susceptibility Testing (EUCAST).Results: According to the ADT results of 3422 E. coli strains included in the study, the antibiotics with the highest resistance rates were respectively; ampicillin (67.1%), cefuroxime axetil (44.9%) amoxicillin/clavulanic acid (42.5%), trimethoprim/sulfamethoxazole (41.7%), ceftazidime (39.6%) and ciprofloxacin (39.7%) were determined. The antibiotics with the lowest resistance rates, respectively; carbapenems (imipenem 0.6%, meropenem 0.6%, ertapenem 2.7%), fosfomycin (2.5%), nitrofurantoin (2.5%) and amikacin (6.3%).Conclusion: The resistance rates detected in this study were found to be similar to those in our country. We believe that these data will guide the treatment of E. coli infections and contribute to the success of treatment.
  • Küçük Resim Yok
    Öğe
    DISTRIBUTION AND ANTIFUNGAL SENSITIVITIES OF CANDIDA SPECIES ISOLATED FROM BLOOD CULTURES: AN 8-YEAR RETROSPECTIVE
    (Nobel Ilac, 2025) Gunduz, Ayten; Mansur, Ahmet
    Objective: Our study aimed to retrospectively examine the species distributions and antifungal susceptibilities of Candida strains isolated from blood cultures. Material and Method: Blood samples received at Malatya Turgut Ozal University Medical Faculty Training and Research Hospital Microbiology Laboratory between January 2015 and December 2022 were loaded into BACTEC (BectonDickinson, USA) and RENDER BC (Zhuhai, China) automated blood culture devices. The identification and antifungal susceptibilities of 292 Candida strains isolated from growing plates were studied with the VITEK 2 Compact (R) (bioMerieux, France) automated system. Antifungal susceptibility results were interpreted in accordance with Clinical & Laboratory Standards Institute (CLSI) recommendations. Results: Among a total of 292 Candida strains, Candida albicans (C. albicans) was isolated most frequently (42.5%), followed by C. parapsilosis (21.1%). 58.6% of the strains belonged to male patients and 41.4% belonged to female patients. 85.6% of the samples came from intensive care clinics and 14.4% from other clinics. C. albicans was most frequently isolated from intensive care clinics (39.0%), and C. parapsilosis was most frequently isolated from other clinics (4.5%). The highest antifungal resistance was detected in fluconazole (9.3%) and voriconazole (5.7%), while the lowest resistance was found in micafungin (2.5%). At the species level, fluconazole resistance was found high in C. albicans (4.8%) and C. parapsilosis (11.3%), and caspofungin resistance was found high in C. glabrata (13.1%). Conclusion: We believe that the regional species distribution and resistance profile information obtained in our study will be guiding in the regulation of treatment procedures.
  • Küçük Resim Yok
    Öğe
    Evaluation of hypoxia pathway genes and serum parameters in new coronavirus pneumonia (COVID-19)
    (Elsevier, 2025) Oterkus, Mesut; Pala, Mukaddes; Yilmaz, Senay Gorucu; Tanriverdi, Elif Seren; Gunduz, Ayten; Delen, Leman Acun; Doger, Cihan
    Background: Coronavirus disease-2019 (COVID-19) causes severe hypoxemia. Unlike normal pneumonia, pneumonia due to COVID-19 causes oxygen deprivation without breathing difficulties (i.e., silent hypoxia). We evaluated the relationship between COVID-19 and hypoxemia and examined possible mechanisms of pneumonia from the perspective of gene expression (HIF1A, vascular endothelial growth factor [VEGF], NF-kB, MEKK1, and EGFR) using real-time PCR and ELISA for serum parameters. Methods: We evaluated 100 individuals (50 patients and 50 controls). The patients were individuals with respiratory symptoms and pneumonia who were (COVID)-D-center dot-19 positive. The relative quantification of standardized samples wa s calculated according to the formula 2-Delta Delta CT. Receiver operating curve (ROC) analysis was made to define the diagnostic power of the genes. The expression changes of four genes in the hypoxia pathway were significant (excluding VEGF) and upregulated in the patients' serums. Results: The fold change values of the HIF1A, VEGF, NF-kB, MEKK1, and EGFR genes were 0.048, 0.688, 0.168, 0.207, and 0.171, respectively, in the cases checked against to the controls. The areas under the ROC values indicating the diagnostic power of the genes were 0.727, 0.538, 0.815, 0.734, and 0.936, respectively. Some serum parameters were significant (age, PCR, urea, LDH, WBC, ferritin, and pO2). Conclusions: The upregulation of some genes in the hypoxia pathway in COVID-19 pneumonia shows that these genes and protein products are candidates for treatment targets. At the same time, the high discriminative power of two genes (NF-kappa B and EGFR) in patients compared to controls indicates their diagnostic potential in serum samples.
  • Küçük Resim Yok
    Öğe
    mTOR signaling pathway genes effect in COVID-19 infection
    (Inonu University, 2024) Pala, Mukaddes; Yılmaz, Şenay Görücü; Tanriverdi, Elif Seren; Gunduz, Ayten; Delen, Leman Acun; Ozturk, Dilara Altay; Öterkuş, Mesut
    Coronavirus disease 2019 (Covid-19) is an infectious disease that causes severe acute respiratory illness caused by coronavirus 2 (SARS-CoV-2). SARS-CoV-2 uses host-specific metabolic pathways, including mTOR. The mTOR pathway is hyperactive in viral respiratory tract infections and contributes positively to viral replication. 100 samples were evaluated, 50 patients (Female=23, Male=27), and 50 controls (Female=29, Male=21). The patients were individuals who were COVID-19 positive. We detected expression changes of 5 genes in mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2) (MLST8, mTOR, RPTOR, MAPKAP1 and RICTOR). Serum samples were obtained from all patients. The expression changes of mTORC1 and mTORC2 Complex genes were evaluated with Real-time PCR method. Receiver operating curve (ROC) analysis was performed to define the diagnostic power of these genes. Expression changes of five genes in the mTORC1 and mTORC2 complex were statistically significant (p =0.001) and upregulated in serum. The area under the ROC Curve values indicating the diagnostic power of genes were 0.948, 0.771, 0.851, 0.798, and 0.805, respectively. These genes may be candidate for treatment targets. The high discriminative power of these genes in patients from controls indicates their diagnostic potential in serum samples.
  • Küçük Resim Yok
    Öğe
    quency of positive samples collectively indicated that the frequencies of both pathogens substantially reduced during the pandemic because of infection control measures. Conclusion: The fact that the COVID-19 pandemic forces us to comply with infection control rules and change our lifestyle shows us that these rules are very important not only in controlling the pandemic, but also in controlling other viral infections.
    (Bilimsel Tip Publishing House, 2022) Duman, Yucel; Yakupogullari, Yusuf; Gunduz, Ayten
    Objective: The coronavirus disease 2019 pandemic has caused many changes in our lifestyle, such as the flow of our daily lives, communication between individuals, traveling, eating, and drinking habits. These changes have reduced the risk of viral infections. In this study, it was aimed to examine the changes in the frequency of rotavirus and enteric adenovirus in children due to changes in lifestyle such as hand hygiene, physical distance and eating habits in individuals before and during the COVID-19 pandemic.Material and Methods: We analyzed data obtained from the laboratory electronic records of hospitals in our region to assess the changes of rotavirus and adenovirus frequency and number of patients admitted to the hospital during the COVID-19 pandemic period compared to pre-pandemic years. Rotavirus and enteric adenovirus antigens were studied using a qualitative immunochromatographic method. Results: Between January 2018 and July 2021, a total of 68.504 patient samples were tested, 60.513 (88.3%) before the COVID-19 pandemic and 7.991 (11.7%) during the pandemic. Mean age of the patients was deter-mined as 4.7 years. Before the pandemic, a total of 6.815 (11.3%) sam-ples for rotavirus and 1.873 (3.1%) samples for adenovirus were found positive. During the pandemic period, a total the 598 (7.5%) samples for rotavirus and 164 (2%) samples for adenovirus were found positive. Monthly median test numbers for rotavirus and adenovirus significant-ly fell about 75% (1.926 vs 493, p< 0.001). In addition, monthly median positivity rates of rotavirus (10.9% vs 7.1%, p= 0.002) and adenovirus (2.9% vs 1.8%, p< 0.001) reduced about 35% and 38%, respectively, after the onset of the pandemic as compared to the pre-pandemic years. The decrease in mean monthly test numbers and the reduction in the fre- quency of positive samples collectively indicated that the frequencies of both pathogens substantially reduced during the pandemic because of infection control measures.Conclusion: The fact that the COVID-19 pandemic forces us to comply with infection control rules and change our lifestyle shows us that these rules are very important not only in controlling the pandemic, but also in controlling other viral infections.