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    Association of 5-HT1A and 5-HT1B Gene Polymorphisms with Obsessive-Compulsive Disorder in a Turkish Population
    (Kure Iletisim Grubu A S, 2016) Aldemir, Secil; Acar, Muradiye; Ocak, Zeynep; Dalbudak, Ercan; Yigitoglu, Muhammet Ramazan; Gunduz, Esra
    Objective: Obsessive-compulsive disorder (OCD) is a frequent neuropsychiatric disorder, in which genetic factors play important causative roles. We investigated the roles of the (-1019 C/G) promoter region polymorphism of 5-HTR1A and the G861C coding region polymorphism of 5-HTR1B genes in susceptibility to OCD in a Turkish population. Methods: Two single nucleotide polymorphisms, 5-HTR1A (rs6296) and 5-HTR1B (rs6295) genes were genotyped in 76 OCD patients and 57 healthy controls that were unrelated, using PCR-RFLP method. Results: We did not observe any difference in the genotype distributions of rs6296 and rs6295 between the OCD patient and control groups. Conclusions: As far as we know, our study is the first to establish the association of genetic variants 5-HTR1A (rs6296) and 5-HTR1B (rs6295) with OCD in a Turkish population. Based on our results, the relationship between polymorphisms of 5-HTR1A (rs6296) and 5-HTR1B (rs6295) with OCD do not seem.
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    Serum and aqueous xanthine oxidase levels, and mRNA expression in anterior lens epithelial cells in pseudoexfoliation
    (Springer, 2015) Simavli, Huseyin; Tosun, Mehmet; Bucak, Yasin Y.; Erdurmus, Mesut; Ocak, Zeynep; Onder, Halil I.; Acar, Muradiye
    The aim of this study was to determine serum and aqueous xanthine oxidase (XO) levels, and mRNA expression in anterior lens epithelial cells in pseudoexfoliation (PEX). In this prospective study, serum, aqueous and anterior lens capsules were taken from 21 patients with PEX and 23 normal subjects who had undergone routine cataract surgery. Serum and aqueous XO levels were analyzed using the colorimetric method. mRNA expression of XO in anterior lens epithelial cells was evaluated using reverse transcription polymerase chain reaction analysis. Serum XO levels (means +/- standard deviations) were 207.0 +/- 86.1 IU/mL and 240.6 +/- 114.1 IU/mL in the normal and PEX groups, respectively (p = 0.310). Aqueous XO levels (means +/- standard deviations) were 65.5 +/- 54.3 IU/mL in the normal group and 130.5 +/- 117.4 IU/mL in the PEX group (p = 0.028). There was a 2.9 fold decrease in mRNA expression in anterior lens epithelial cells of PEX, which is significantly lower than the normal group (p = 0.01). Higher aqueous XO levels lacking associated different serum XO suggests higher oxidative stress in the aqueous. Higher aqueous XO levels in PEX with decreased mRNA expression in anterior lens epithelial cells indicate possible overexpression of XO in other structures related to the aqueous.
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    The effects of hypericin on ADAMTS and p53 gene expression in MCF-7 breast cancer cells
    (Imprimatur Publications, 2014) Acar, Muradiye; Ocak, Zeynep; Erdogan, Kubra; Cetin, Elif Nihan; Hatipoglu, Omer Faruk; Uyeturk, Ummugul; Gunduz, Esra
    Purpose: The purpose of this study was to determine the effects of hypericin on MCF-7 (Michigan Cancer Foundation-7) breast cancer cells, as it is known to exert an antitumor effect on the expression and regulation of ADAMTS1, 3, 10 and the p53 gene in breast cancer cells. Methods: MFC-7 cells were cultured and subjected separately to various doses (1, 5 and 7.5 mu g/mL) hypericin. After 24 hrs, RNA was isolated and transcribed into cDNA. Expression analysis was performed by real time (RT)-PCR and cell survival was determined by the XTT assay. Results: While the expression of ADAMTS1 in MFC-7 cells decreased to 0.04-fold after exposure to 1 mu g/mL hypericin, the expression increased by 5.6- and 36-fold with 5 and 7.5 mu g/mL, respectively. Furthermore, ADAMTS3 expression in MCF7 cells increased 3.9-fold with the use of 5 mu g/mL of hypericin. These concentrations of hypericin did not lead to significant changes in the expression of ADAMTS10 and the p53 gene. Viability of cancer cells as evaluated by the XTT assay showed that hypericin concentration of 7.5 mu g/mL led to increased apoptosis of cancer cells. Conclusion: The increase in ADAMTS1 expression may prevent metastasis or facilitate the development of an adjuvant factor with tumor-suppressive effects. Hypericin may therefore exert its antitumor and apoptotic effects in MFC-7 cells via ADAMTS1 and ADAMTS3.