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Öğe Investigation of genetic structures of coloured horses by mtdna d-loop sequence analysis in Turkey(Kafkas Üniversitesi, 2019) Köseman, Abdurrahman; Özşensoy, Yusuf; Erdoǧan, Metin; Yaralı, Cevdet; Toprak, Burhan; Zengin, Kemal; Şeker, İbrahimThe aim of this study was to determine the genetic structure of Coloured horses in Turkey by analysis of the D-loop sequence of mitochondrial DNA (mtDNA). A total of 28 Coloured horses were examined. DNA was extracted from blood samples using DNA isolation kit; 519 bp long mtDNA D-loop region was amplified by PCR and sequenced by capillary electrophoresis system. Population parameters and phylogenetic trees were drawn by using MEGA4 software package. It was also compared with the DNA sequences of horse populations from diffrent countries. In this study, 42 diffrent polymorphism regions and 10 diffrent haplogroups were detected. Additionaly, Tajima D value was found to be -0.31 and population expansion was determined. It was determined that the base diffrences among the horses ranged between 0.000 and 0.032. It was detected that the horses formed diffrent clusters from each other and they were intertwined with the populations of diffrent countries. Moreover, it was also observed that some horses formed their own populations at diffrent points from the other countries’ horses. As a result, it was observed that the genetic structures of horses which used in population were diffrent from each other and they originated from diffrent mothers. According to the results obtained, it is considered that Coloured horses can be a native horse breed of Turkey.Öğe Investigation of Genetic Variations using Microsatellite Markers in Colored Horses in Turkey(Pleiades Publishing, 2020) Köseman, Abdurrahman; ÖzŞensoy, Yusuf; Erdoǧan, Metin; Yaralı, Cevdet; Toprak, Burhan; Zengin, Kemal; Şeker, İbrahimAbstract: The aim of this study was to determine the genetic variation of Colored horses by using microsatellite markers. In the study, samples from a total of 28 Colored horse were genotyped using 17 microsatellite markers, 12 of them are recommended by the International Society for Animal Genetics (ISAG) and the other 5 loci included by the StockMarks Genotyping Kit. A total of 129 different alleles were identified and the average number of alleles was 7.588.The heterozygosity value (Ho) was 0.286 as the lowest (LEX3) and 0.929 (HTG10) as the highest among the markers; the expected lowest heterozygosity value (He) was determined as 0.607 (HTG4) and the highest one was 0.876 (VHL20). The mean Ho and He values were calculated as 0.729 and 0.771, respectively. On a population basis, the mean FIS and FIT values were 0.047. The mean PIC value was 0.741. In the phylogenetic tree formed by the neighbour joining tree (NJT), it was determined that the horses were generally categorized into 3 groups. Although statistically significant results (P = 0.01008) were found in the studies investigating whether the population of Colored horses had the risk of extinction or not, it was determined that the data showed normal L distribution indicating no risk of recent extinction. It was determined that the Colored horses had a genetically important potential.Öğe Parentage verification and genetic diversity of the Arabian and Thoroughbred horse populations in Türkiye using microsatellite analysis(Gesellschaft Schweizer Tierarztinnen & Tierarzte, 2023) Yarali, Cevdet; Ozsensoy, Yusuf; Koseman, Abdurrahman; Seker, Ibrahim; Toprak, Burhan; Zengin, KemalThis study aimed to determine the effectiveness of parentage verification in Arabian and Thoroughbred horses in Turki-ye using microsatellite markers. A total of 813 Arabian and 959 Thoroughbred horses were genotyped using a total of 17 microsatellite markers. The mean effective number of alleles was 3,34 and the mean number of alleles was 7,41 in Arabian horses. It was calculated that the mean He and Ho values in Arabian horses were 0,677 and 0,680, respective-ly. The mean effective number of alleles was 3,55 and the mean number of alleles was 6,59 in Thoroughbred horses. It was calculated that the mean Ho and He values in Thor-oughbred horses were 0,697 and 0,684, respectively. When the studied Arabian and Thoroughbred horse populations are considered as a single population, the mean FIT, FST and FIS values were found to be 0,063, 0,074 and, - 0,011, re-spectively. Also, 4 loci in Arabian horses and 3 loci in Thor-oughbred horses significantly deviated from HWE. The mean PIC value was 0,63 in Arabian horses and 0,64 in Thoroughbred horses. As a result; the microsatellites includ-ing the most informative 15 and 9 loci had a total value of > 0,9999 (11 and 7 loci > 0,999) in each population for PE - 1 and PE - 3, respectively. It has been concluded that parentage verification and genetic identification can be made successfully in the Arabian and Thoroughbred horse populations by using the microsatellite markers panel.Öğe Phylogenetic and bottleneck analysis of the Turkish Arabian and Thoroughbred horse populations(Univ Zulia, Facultad Ciencias Veterinarias, 2023) Yarali, Cevdet; Koseman, Abdurrahman; Ozsensoy, Yusuf; Seker, Ibrahim; Toprak, Burhan; Zengin, KemalThis study aimed to determine the phylogenetic and bottleneck analyses of Turkish Thoroughbred and Arabian horse populations. In the study, genotyping was performed using a total of 17 microsatellite markers in the samples taken from 959 Thoroughbred and 813 Arabian horses. The average effective allele number in Arabian horses was 3.338 and the average number of alleles was 7.412 in Thorougbred horses. Genetic distance and genetic identity between Thoroughbred and Arabian breeds was 0.411 and 0.663, respectively. Also genetic identity in each Arabian and Thoroughbred horse populations was 1.000. The FCA table showed that the two breeds were completely separated from each other and were compatible. In conclusion; the bottlenecks of Thoroughbred and Arabian horse populations were in a normal L distribution and these horse breeds do not appear to have succumbed to introgression. Therefore, they are not yet at risk of extinction any time soon.
