Investigation of the İn Vitro Effect of Vanillic Acid on Wound Healing via FN1 and COL1?1 Genes

Basit Öğe Kaydı
dc.contributor.authorYeni, Yeşim
dc.date.accessioned2025-10-24T18:04:19Z
dc.date.available2025-10-24T18:04:19Z
dc.date.issued2024
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractObjective: Wound healing is characterized by the removal of dead/damaged tissue, the formation of new tissue, and finally, the restoration of the damaged tissue to its original function, starting from the moment of tissue injury. Vanillic acid (VA) is an important component of wheat bran and can heal wounds thanks to its antioxidant potential. This work aimed to investigate the dose-dependent effects of VA (1-2-4-8-16 and 32 ?g/ml) in an in vitro way using a wound healing pattern in fibroblast cells. Methods: The MTT test was performed to determine cell viability 48 hours after VA application to the cells in which the wound model was created (except for the control and wound groups). The cells were examined morphologically with an inverted microscope. ELISA and Real-Time PCR analyses were performed to determine changes in oxidative stress parameters and FN1 and COL1?1 gene expressions. Results: The highest percentage closure rate of fibroblast cells in the in vitro wound pattern analysis and the highest percentage of cell viability by MTT analysis were determined in the VA-32 treated group. Morphological images showed that the evaluated gene expressions increased in fibroblast cells in a VA dose-dependent manner. Conclusion: Our findings demonstrated for the first time that VA promotes cell migration and proliferation by regulating oxidative stress and FN1A and COL1?1 genes. The results of this work are thought to pioneer the use of VA in in vivo wound healing studies.
dc.identifier.doi10.5798/dicletip.1501313
dc.identifier.endpage240
dc.identifier.issn1300-2945
dc.identifier.issn1308-9889
dc.identifier.issue2
dc.identifier.startpage233
dc.identifier.trdizinid1241836
dc.identifier.urihttps://doi.org/10.5798/dicletip.1501313
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1241836
dc.identifier.urihttps://hdl.handle.net/20.500.12899/2792
dc.identifier.volume51
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofDicle Tıp Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzTR-Dizin_20251023
dc.subjectHücre ve Doku Mühendisliği
dc.subjectBiyoteknoloji ve Uygulamalı Mikrobiyoloji
dc.subjectSağlık Bilimleri ve Hizmetleri
dc.subjectBiyokimya ve Moleküler Biyoloji
dc.subjectHücre Biyolojisi
dc.subjectMTT
dc.subject8-OHdG
dc.subjectFibroblast
dc.subjectVanillic acid
dc.titleInvestigation of the İn Vitro Effect of Vanillic Acid on Wound Healing via FN1 and COL1?1 Genes
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu