Evaluating DNA barcoding using cpDNA matK and rbcL for species identification and phylogenetic analysis of Prunus armeniaca L. (Rosaceae) genotypes

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dc.contributor.authorSevindik, Emre
dc.contributor.authorKorkom, Yunus
dc.contributor.authorMurathan, Zehra Tugba
dc.date.accessioned2026-06-19T06:41:07Z
dc.date.available2026-06-19T06:41:07Z
dc.date.issued2024
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractIn this study, DNA barcoding and phylogenetic analysis of five Prunus armeniaca L. genotypes (Cataloglu, Hacihaliloglu, Hasanbey, Hudayi, and Kabaasi) grown in Malatya/Turkiye were conducted using chloroplast DNA (cpDNA) matK and rbcL regions. The cpDNA matK region was amplified using matK472F and matK1248R primers, while the rbcL region was amplified with rbcLaF and rbcLaR primers. The matK and rbcL sequences were utilized to assess nucleotide ratios, genetic distance, and nucleotide diversity (p). The neighbor- joining (NJ) tree including other Prunus species from NCBI was created. In addition, physiochemical and 3-D analysis of matK and rbcL proteins were performed. As a result, p = 0.000549 for matK sequence and p = 0.002657 for rbcL sequence were determined. NJ (neighbor joining) phylogenetic trees formed with both matK and rbcL sequences were found to be compatible with each other. The matK and rbcL gene regions were found to be suitable for phylogenetic analysis.
dc.identifier.doi10.1007/s10722-023-01748-9
dc.identifier.endpage1835
dc.identifier.issn0925-9864
dc.identifier.issn1573-5109
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85172126962
dc.identifier.scopusqualityQ2
dc.identifier.startpage1825
dc.identifier.urihttps://doi.org/10.1007/s10722-023-01748-9
dc.identifier.urihttps://hdl.handle.net/20.500.12899/6084
dc.identifier.volume71
dc.identifier.wosWOS:001073514300002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofGenetic Resources and Crop Evolution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260612
dc.subjectMatk
dc.subjectRbcl
dc.subjectPhylogenetic
dc.subjectPrunus Armeniaca
dc.subjectTurkiye
dc.titleEvaluating DNA barcoding using cpDNA matK and rbcL for species identification and phylogenetic analysis of Prunus armeniaca L. (Rosaceae) genotypes
dc.typeArticle

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