Investigation of the effects of 5-fluorouracil and thymoquinone on head and neck cancer stem cells

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dc.authoridocak, tarik/0000-0003-4721-8616|acar, muradiye/0000-0003-4357-5229;
dc.contributor.authorAcar, Muradiye
dc.contributor.authorGunduz, Mehmet
dc.contributor.authorOcak, Tarik
dc.contributor.authorGurses, Hacer Esra
dc.contributor.authorCetin, Elif Nihan
dc.contributor.authorCeylan, Furkan Sacit
dc.contributor.authorSefa, Alperen
dc.date.accessioned2025-10-24T18:10:30Z
dc.date.available2025-10-24T18:10:30Z
dc.date.issued2015
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractPurpose: A subpopulation of cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC) play a critical role in invasion, metastasis, and tumour recurrence post-therapy. 5-Fluorouracil (5-FU) is a pyrimidine analogue that is used in the treatment of HNSCC. Thymoquinone (TQ), found in Cumin (Nigella sativa) seed oil, has anti-diabetic, anti-oxidant, anti-inflammatory, and antitumour effects. The purpose of this study was to examine the effects of 5-FU and TQ on CSCs. Methods: ALDH1 (+) cells were isolated from the HNSCC cell line UT-SCC-74A using magnetic activated cell sorting (MACS) and sphere formation was confirmed. Real-time RTPCR revealed was used to measure expression levels of a number of stem cell markers, including OCT4, SOX2, and KLF-4. To investigate the effects of the therapeutic agents, 5-FU and TQ, concentration-dependent assay procedures were used and cell viability after treatment was measured using XTT assay. Results: ALDH1 (+) cells formed spheres while the ALDH1 (-) cells did not. Real-time RT-PCR revealed that a number of stem cell markers, including OCT4, SOX2, and KLF-4, were up-regulated in the CSCs. XTT results showed that, when given alone at a dose of 50 ng/mL, neither agent had a statistically significant cytotoxic effect on CSCs. However, when dosed in combination, TQ and 5-FU had a synergistic effect on CSC cytotoxicity. Conclusion: We report the effects of TQ and 5-FU on CSCs in HNSCC.
dc.identifier.endpageE213
dc.identifier.issn0147-958X
dc.identifier.issn1488-2353
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84939126099
dc.identifier.scopusqualityQ2
dc.identifier.startpageE209
dc.identifier.urihttps://hdl.handle.net/20.500.12899/4220
dc.identifier.volume38
dc.identifier.wosWOS:000359119100011
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCanadian Soc Clinical Investigation
dc.relation.ispartofClinical And Investigative Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectCarcinoma
dc.titleInvestigation of the effects of 5-fluorouracil and thymoquinone on head and neck cancer stem cells
dc.typeArticle

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