First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye

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dc.contributor.authorusta, mustafa
dc.contributor.authorgüller, abdullah
dc.contributor.authorSipahioglu, Hikmet Murat
dc.date.accessioned2025-10-24T18:03:46Z
dc.date.available2025-10-24T18:03:46Z
dc.date.issued2023
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractPhytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic and three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a template for PCR reactions. The phytoplasma agents were confirmed in the two symptomatic samples. BLASTn search of 16S rRNA of two sequences shared identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’. Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is identical to the reference pattern of the 16SrII-D subgroup, with a similarity coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D subgroup. This is the first report of tomato witches' broom disease related to 16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye.
dc.identifier.doi10.29133/yyutbd.1205091
dc.identifier.endpage63
dc.identifier.issn1308-7576
dc.identifier.issn1308-7584
dc.identifier.issue1
dc.identifier.startpage54
dc.identifier.trdizinid1162748
dc.identifier.urihttps://doi.org/10.29133/yyutbd.1205091
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1162748
dc.identifier.urihttps://hdl.handle.net/20.500.12899/2420
dc.identifier.volume33
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofYüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzTR-Dizin_20251023
dc.subjectMikrobiyoloji
dc.subjectZiraat Mühendisliği
dc.subjectBitki Bilimleri
dc.subjectSolanum lycopersicum
dc.subject16S rRNA
dc.subjectRFLP
dc.subjectmolecular phylogeny
dc.subjectphloem restricted bacteria
dc.titleFirst Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye
dc.typeArticle

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