Molecular Genetic Diversity and Phylogenetic Investigation of Pyrus communis L. (Rosaceae) Genotypes Using cpDNA Sequences with RAPD and ISSR Analyses

Basit Öğe Kaydı
dc.contributor.authorSevindik, Emre
dc.contributor.authorEfe, Fadime
dc.contributor.authorMurathan, Zehra Tugba
dc.date.accessioned2026-06-19T06:41:15Z
dc.date.available2026-06-19T06:41:15Z
dc.date.issued2023
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractIn this study, molecular genetic diversity and phylogenetic investigation of seven Pyrus communis L. genotypes were conducted based on random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence repeat PCR (ISSR-PCR) using chloroplast DNA trnL intron and trnL-F sequences. Pyrus communis genotypes were obtained from Ardahan province, Turkiye. A total of 15 RAPD and 18 ISSR primers were used to determine the molecular genetic diversity of the Pyrus communis genotypes. For trnL intron, trnC, trnD, for trnL-F, trnE, and trnF primers were used in PCR for amplification. In all, 57 and 91 bands were obtained by RAPD and ISSR analysis, respectively. Polymorphism rates were 78.94% and 85.71%, respectively. For trnL intron analyses, the average nucleotide composition consisted of 39% thymine, 17.5% cytosine, 28.4% adenine, and 15.1% guanine. In trnL-F assays, the average nucleotide composition consisted of 31.9% thymine, 14.4% cytosine, 37.5% adenine, and 16.1% guanine. The results of the phylogenetic tree constructed with trnL intron and trnL-F sequences of Alchemilla, Rosa, Sorbus, Malus, Prunus, and Cotoneaster species of the Rosaceae family obtained from NCBI were found to be similar. As a result of the study, polymorphisms were obtained at the rates of 78.94% and 85.71% from RAPD and ISSR analyses, respectively. In conclusion, detailed phylogenetic relationships of pear genotypes using chloroplast trnL intron and trnL-F sequences with respect to other species from the same family were revealed.
dc.description.sponsorshipTUBITAK [1919B011803030]
dc.description.sponsorshipThis research was supported by the TUBITAK 2018/2209-A (Project no: 1919B011803030).
dc.identifier.doi10.1007/s10341-022-00798-0
dc.identifier.endpage240
dc.identifier.issn0014-0309
dc.identifier.issn1439-0302
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85145210773
dc.identifier.scopusqualityQ2
dc.identifier.startpage231
dc.identifier.urihttps://doi.org/10.1007/s10341-022-00798-0
dc.identifier.urihttps://hdl.handle.net/20.500.12899/6125
dc.identifier.volume65
dc.identifier.wosWOS:000905846500004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofErwerbs-Obstbau
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260612
dc.subjectMolecular Marker
dc.subjectPear
dc.subjectTrnl Intron
dc.subjectTrnl-F
dc.subjectTurkiye
dc.titleMolecular Genetic Diversity and Phylogenetic Investigation of Pyrus communis L. (Rosaceae) Genotypes Using cpDNA Sequences with RAPD and ISSR Analyses
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu