The distribution of allele frequencies of the 15 Short Tandem Repeats loci in Elazığ population, Türkiye

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dc.contributor.authorAksoy, Aziz
dc.contributor.authorTOKDEMİR, MEHMET
dc.contributor.authorkonar, vahit
dc.date.accessioned2025-10-24T18:04:16Z
dc.date.available2025-10-24T18:04:16Z
dc.date.issued2024
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractThe DNA within the Human Genome involves loci with high polymorphism such as short tandem repeats (STR). The STR polymorphism analysis is extensively used for numerous purposes such as identification studies in forensic sciences, paternity tests, genome mapping, and population studies. In this study, blood and swab samples were obtained from 200 unrelated healthy individuals living in Elazig province. DNA was amplified via the AmpFlSTR Identifiler PCR Amplification Kit (Applied Biosystems, USA) to analyze the 15 STR loci. Using the ABI PRISM310 Genetic Analyzer capillary electrophoresis, the samples were analyzed and the allelic ladder was used to compare and identify the alleles of the samples, the distribution of the allele frequencies and peak values were examined. For statistical analysis Arlequin v.3.11 and PowerStats v.1.2 were used to assess the data. To determine if the allele frequencies were compatible with the Hardy-Weinberg (HW) equilibrium, the Markov chain and the Fisher's exact test p-value (p<0.05) were examined. It was determined that the D8S1179 (0.00002), D21S11 (0.00593) and vWA (0.04939) loci were not compatible with the HW equilibrium, but the rest of the loci were compatible. For the loci, the combined power of exclusion is 0.9999995, the combined power of discrimination is 0.9999999999998. The probability of matching for the 15 STR loci was calculated to be 1 in 1.364x10 ?17. The power of discrimination was quite high when all the loci were considered. The results of this study indicate that the populations residing in the Elazığ province may benefit from paternity testing, forensic identification, and genomic mapping using the data as an appropriate marker.
dc.identifier.doi10.5455/medscience.2024.08.092
dc.identifier.endpage768
dc.identifier.issn2147-0634
dc.identifier.issue4
dc.identifier.startpage763
dc.identifier.trdizinid1304391
dc.identifier.urihttps://doi.org/10.5455/medscience.2024.08.092
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1304391
dc.identifier.urihttps://hdl.handle.net/20.500.12899/2748
dc.identifier.volume13
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofMedicine Science
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzTR-Dizin_20251023
dc.subjectPolymorphism
dc.subjecthuman identification
dc.subjectShort tandem repeat
dc.subjectAmpflSTR
dc.subjectgenetic analyzer
dc.titleThe distribution of allele frequencies of the 15 Short Tandem Repeats loci in Elazığ population, Türkiye
dc.typeArticle

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