Role of miR-200c/miR-141 in the regulation of epithelial-mesenchymal transition and migration in head and neck squamous cell carcinoma

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dc.contributor.authorTamagawa, Shunji
dc.contributor.authorBeder, Levent Bekir
dc.contributor.authorHotomi, Muneki
dc.contributor.authorGunduz, Mehmet
dc.contributor.authorYata, Kazuya
dc.contributor.authorGrenman, Reidar
dc.contributor.authorYamanaka, Noboru
dc.date.accessioned2025-10-24T18:09:47Z
dc.date.available2025-10-24T18:09:47Z
dc.date.issued2014
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractEpithelial-mesenchymal-transition (EMT) is a critical step in tumor invasion and metastasis, while its fate is mainly defined by the balanced expression between the miR-200 family and ZEB transcription factors. In this study, we observed a reciprocal correlation between miR-200c/mir-141 and ZEB1, as well as between ZEB2 and E-cadherin expression in a panel of 13 head and neck squamous cell carcinoma (HNSCC) cell lines. We also confirmed that the enforced expression of miR-200c and miR-141 significantly reduced the migration capacity of HNSCC cells. Accordingly, the enforced expression of miR-200c and mir-141 resulted in a significant upregulation in E-cadherin expression, contrary to the significant downregulation in ZEB1 expression in 3 cell lines (UTSCC-24A, UTSCC-24B and UTSCC-6A cells). Another pair of cell lines, UTSCC-60A and UTSCC- 60B failed to show a significant change in the expression of E-cadherin or ZEB1/ZEB2 during the enforced expression of miR-200c/miR-141. To address the issue, we focused on the hypermethylation status of the ZEB1/2 promoters, which have both been shown to include wide CpG islands. We observed a marked upregulation in both ZEB1 and ZEB2 mRNA expression following treatment with a demethylating agent in both pairs of UTSCC cell lines. In conclusion, our findings confirm the existence of a reciprocal correlation between the mir-200 family and the ZEB family, and demonstrate the role of the miR-200 family in EMT, as well as in the migration and invasion ability of HNSCC cells. Furthermore, our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis.
dc.description.sponsorshipMinistry of Education, Culture, Sports, Science and Technology of Japan
dc.description.sponsorshipWe thank Dr Hirohashi for his comments and suggestions regarding the manuscript. This study was partially presented at the 8th International Conference on Head and Neck Cancer. This study was partially supported by Grants-in-Aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to S. T. and L.B.B.).
dc.identifier.doi10.3892/ijmm.2014.1625
dc.identifier.endpage886
dc.identifier.issn1107-3756
dc.identifier.issn1791-244X
dc.identifier.issue4
dc.identifier.pmid24424572
dc.identifier.scopus2-s2.0-84899529221
dc.identifier.scopusqualityQ1
dc.identifier.startpage879
dc.identifier.urihttps://doi.org/10.3892/ijmm.2014.1625
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3830
dc.identifier.volume33
dc.identifier.wosWOS:000334313000016
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofInternational Journal Of Molecular Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20251023
dc.subjecthead and neck; miR-200 family; epithelial-mesenchymal transition
dc.titleRole of miR-200c/miR-141 in the regulation of epithelial-mesenchymal transition and migration in head and neck squamous cell carcinoma
dc.typeArticle

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