Comparison of manual and automated nucleic acid isolation methods for HBV-DNA and HCV-RNA assays

Basit Öğe Kaydı
dc.contributor.authorYağmur, Gülhan
dc.contributor.authorAltun, Hatice Uludağ
dc.contributor.authorGökahmetòğlu, Selma D.
dc.contributor.authorBasok, Ela
dc.date.accessioned2025-10-24T18:06:52Z
dc.date.available2025-10-24T18:06:52Z
dc.date.issued2015
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractIn the diagnosis and monitoring of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, it is important to use methods that can provide rapid and reliable results. The present study aimed to compare the automated and manual extraction methods during the nucleic acid isolation phase for HBV-DNA and HCVRNA assays. The study included 93 serum samples, 49 of which were for the HBV-DNA assay and 44 for the HCVRNA assay. DNA and RNA isolation from the samples was performed manually with a “QIAmpMin Elute Kit” (Qiagen, Germany) and the automated isolation system, NucliSens easyMAG (BioMérieux, France). All the extraction products were amplified using the iCycler device (Bio-Rad, USA). With both methods, compliance was found in 21 (42.8%) samples in the HBV-DNA assay; nine (18.3%) samples had a higher amount of viral nucleic acid with the manual method, whereas 19 samples (38.7%) were found to have a higher amount of nucleic acid with the automated system. For the HCV-RNA assay, total compliance was found in 31 (70.4%) samples; 12 (27.2%) samples had a higher amount of viral nucleic acid with the manual method whereas one sample (2.2%) was found to have a higher amount of nucleic acid with the automated system. It was concluded that the NucliSens easy- MAG automated isolation system can be used with confidence for nucleic acid extraction due to its higher sensitivity, providing results in a shorter time, and assured standardization. © 2021 Elsevier B.V., All rights reserved.
dc.identifier.endpage252
dc.identifier.issn1124-9390
dc.identifier.issue3
dc.identifier.pmid26397294
dc.identifier.scopus2-s2.0-84943249104
dc.identifier.scopusqualityQ1
dc.identifier.startpage247
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3248
dc.identifier.volume23
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherEDIMES Edizioni Medico Scientifiche
dc.relation.ispartofInfezioni in Medicina
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzScopus_20251023
dc.subjectHBV-DNA
dc.subjectHCV-RNA
dc.subjectNucleic acid isolation
dc.subjectNucliSens easyMAG
dc.titleComparison of manual and automated nucleic acid isolation methods for HBV-DNA and HCV-RNA assays
dc.title.alternativeConfronto di metodi manuali e automatizzati di isolamento per i test HBV-DNA e HCV-RNA
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu