Is phenytoin a safe agent for staple line recovery after gastric sleeve surgery in rats?

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dc.contributor.authorGay, Ferhat
dc.contributor.authorDuran, Ali
dc.contributor.authorTokay, Esra
dc.contributor.authorHacioglu, Nelin
dc.contributor.authorKockar, Feray
dc.contributor.authorAltun, Eren
dc.contributor.authorKanat, Burhan Hakan
dc.date.accessioned2025-10-24T18:09:36Z
dc.date.available2025-10-24T18:09:36Z
dc.date.issued2023
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractBACKGROUND: The most challenging and mortal complication of gastric sleeve surgery (SG) is staple line leakage. Although many agents have been used for increasing tissue healing on the stapler line, there is still no consensus on its effectiveness and efficacy. The aim of study is to determine the effect of phenytoin on the healing process of gastric sleeve surgery in rats. METHODS: On the 10th post-operative day, the effects of phenytoin on bursting pressure in the stapler line were evaluated alongside pathohistological examinations. To investigate the molecular impact of phenytoin on the expression of TGF-8, VEGF, FGF2, and p53 genes, quantitative real-time polymerase chain reaction was utilized. In addition, gene expressions at the protein level were determined by immunohistochemical analysis. RESULTS: No signs of intra-abdominal leakage were observed in the resected samples. A statistically essential extend in stable bursting pressure measure was observed between the control group and the group treated with phenytoin application. Pathohistological results indicate that the mean score of collagens of the study group (3.2 +/- 0.42) was significantly higher than the control group (2.3 +/- 0.48) (P=0.003). In addition, the mean epithelization score of the study group (3.4 +/- 0.52) was significantly higher than the control group (2.1 +/- 0.57) (P=0.001). mRNA of TGF8, FGF2, VEGF, and p53 genes drastically increased phenytoin treated group. High FGF2 protein expression levels were determined from phenytoin use compared to the control group. CONCLUSION: Molecular studies suggest that phenytoin may increase the healing process of Gastric sleeve following SG in rats and may become a new agent for the prevention of human gastric leaks.
dc.identifier.doi10.14744/tjtes.2023.29035
dc.identifier.endpage1328
dc.identifier.issn1306-696X
dc.identifier.issn1307-7945
dc.identifier.issue12
dc.identifier.pmid38073452
dc.identifier.startpage1321
dc.identifier.trdizinid1263572
dc.identifier.urihttps://doi.org/10.14744/tjtes.2023.29035
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1263572
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3731
dc.identifier.volume29
dc.identifier.wosWOS:001125282700007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTurkish Assoc Trauma Emergency Surgery
dc.relation.ispartofUlusal Travma Ve Acil Cerrahi Dergisi-Turkish Journal Of Trauma & Emergency Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20251023
dc.subjectFGF2; Gastric sleeve surgery; p53; Phenytoin; TGF; VEGF.
dc.titleIs phenytoin a safe agent for staple line recovery after gastric sleeve surgery in rats?
dc.typeArticle

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