Cloning and Expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Production
Küçük Resim Yok
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
AMER SCIENTIFIC PUBLISHERS
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study focused on a new plasmid and new recombinant strain developed for the production of microbial chondroitin sulfate a new and limited area of study the strategies we applied for the production of microbial chondroitin sulfate, and the possible contributions of this study to published research literature. In this study, pETM6-PACF, which carries the genes responsible for capsular chondroitin synthesis [kfA, kfoC, kfoF] was used as the basic plasmid. The Vitreoscilla hemoglobin gene region was transformed into this basic plasmid and the common expression of both gene groups was added to research literature for the first time. This plasmid was transferred to non-pathogenic E. coil (C2987) to produce a completely new chondroitin source specific to this study. Following the transformation by chondroitin synthesis, and the subsequent microbial production of chondroitin by the application of purification protocols, microbial chondroitin sulfate was produced in sulfate form. Consequently, in comparison to published literature, a product with a low molecular weight value of 269 Daltons was developed. This product, which has significant potential drug potency, can be used in many different areas as a novel and unique biomedical product.
Açıklama
Anahtar Kelimeler
Chondroitin, Chondroitin Sulfate, Vitreoscilla Hemoglobin, Bacterial Hemoglobin, Vitreoscilla Hemoglobin Gene, Crystal-Structure, Polyscaccharides, Degradation, Acid
Kaynak
SCIENCE OF ADVANCED MATERIALS
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
11
Sayı
12
Künye
Erenler, A. S., Geckil, H., Karabulut, A. B., Akpolat, N., Sevimli, R., Ulke, E., & Aliyeva, A. (2019). Cloning and expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Production. Science of Advanced Materials, 11(12), 1745-1754.
