Cloning and Expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Production

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dc.authorid0000-0002-7873-2805en_US
dc.contributor.authorErenler, Ayşe Şebnem
dc.contributor.authorGeçkil, Hikmet
dc.contributor.authorAkpolat, Nusret
dc.contributor.authorSevimli, Reşit
dc.contributor.authorÜlke, Esra
dc.contributor.authorAliyeva, Aygün
dc.date.accessioned2022-04-12T12:06:07Z
dc.date.available2022-04-12T12:06:07Z
dc.date.issued2019en_US
dc.departmentMTÖ Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractThis study focused on a new plasmid and new recombinant strain developed for the production of microbial chondroitin sulfate a new and limited area of study the strategies we applied for the production of microbial chondroitin sulfate, and the possible contributions of this study to published research literature. In this study, pETM6-PACF, which carries the genes responsible for capsular chondroitin synthesis [kfA, kfoC, kfoF] was used as the basic plasmid. The Vitreoscilla hemoglobin gene region was transformed into this basic plasmid and the common expression of both gene groups was added to research literature for the first time. This plasmid was transferred to non-pathogenic E. coil (C2987) to produce a completely new chondroitin source specific to this study. Following the transformation by chondroitin synthesis, and the subsequent microbial production of chondroitin by the application of purification protocols, microbial chondroitin sulfate was produced in sulfate form. Consequently, in comparison to published literature, a product with a low molecular weight value of 269 Daltons was developed. This product, which has significant potential drug potency, can be used in many different areas as a novel and unique biomedical product.en_US
dc.identifier.citationErenler, A. S., Geckil, H., Karabulut, A. B., Akpolat, N., Sevimli, R., Ulke, E., & Aliyeva, A. (2019). Cloning and expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Production. Science of Advanced Materials, 11(12), 1745-1754.en_US
dc.identifier.doi10.1166/sam.2019.3693
dc.identifier.endpage1754en_US
dc.identifier.issn1947-2935en_US
dc.identifier.issn1947-2943en_US
dc.identifier.issue12en_US
dc.identifier.startpage1745en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12899/972
dc.identifier.volume11en_US
dc.identifier.wosWOS:000490289600012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorBay Karabulut, Aysun
dc.language.isoenen_US
dc.publisherAMER SCIENTIFIC PUBLISHERSen_US
dc.relation.ispartofSCIENCE OF ADVANCED MATERIALSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChondroitinen_US
dc.subjectChondroitin Sulfateen_US
dc.subjectVitreoscilla Hemoglobinen_US
dc.subjectBacterial Hemoglobinen_US
dc.subjectVitreoscilla Hemoglobin Geneen_US
dc.subjectCrystal-Structureen_US
dc.subjectPolyscaccharidesen_US
dc.subjectDegradationen_US
dc.subjectAciden_US
dc.titleCloning and Expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Productionen_US
dc.typeArticleen_US

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