Downregulatory effect of miR-342-3p on epileptogenesis in the PTZ-kindling model

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dc.contributor.authorPala, Mukaddes
dc.contributor.authorMeral, Ismail
dc.contributor.authorAcikgoz, Nilgun Pala
dc.contributor.authorYilmaz, Senay Gorucu
dc.contributor.authorOkur, Semra Karaca
dc.contributor.authorAcar, Seyma
dc.contributor.authorAkbas, Fahri
dc.date.accessioned2026-06-19T06:41:05Z
dc.date.available2026-06-19T06:41:05Z
dc.date.issued2022
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractBackground Epileptogenesis is a process that results in neurons firing abnormally, causing seizures. Increasing evidence has shown that miRNAs expressed in the epileptic hippocampus are involved in epileptogenesis. We demonstrated the expression changes of miRNAs that may be effective in epileptogenesis in silico analysis in the kindling model created with Pentylenetetrazole (PTZ). Thus, we aimed to identify the target genes responsible for epileptogenesis. Methods and results Fifteen male Wistar-albino rats (200-230 g) were randomly divided into two groups control (n = 6) and PTZ (n = 9). The control group received 0.5 ml saline, and the PTZ group (35 mg/kg i.p.) intraperitoneally (i.p.) (11 times, every other day) to induce tonic-clonic seizures. Seizures were observed and scored 30 min after PTZ injection. After the last dose of PTZ (75 mg/kg) administration, the hippocampus tissues of the rats were removed by anesthesia. Analysis of miRNAs was performed with the Affymetrix gene chip miRNA sequence (728 miRNA) and confirmed by the Real-Time Polymerase Chain Reaction (Real-Time PCR) method (29 miRNAs). We evaluated the expression change of the target gene of miRNA, whose expression change was detected using in silico analysis, by q-RT PCR. Eight miRNAs with changes in expression were detected. Of these miRNAs, miR-342-p was downregulated in the PTZ group and was statistically significant (p < 0.005). Ultimately, we determined that the target gene of miR-342-p is a metabotropic glutamate receptor 2 (GRM2) and that GRM2 expression is upregulated. Conclusions Downregulation of miR-342-3p in the PTZ kindling model may result in the upregulation of GRM2.
dc.description.sponsorshipResearch Fund of Bezmialem Vakif University [12.2014/15]
dc.description.sponsorshipThe present work was funded by the Research Fund of Bezmialem Vakif University (grant number 12.2014/15).
dc.identifier.doi10.1007/s11033-022-08017-5
dc.identifier.endpage12006
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue12
dc.identifier.orcid0000-0003-0523-7819
dc.identifier.orcid0000-0002-3837-250X
dc.identifier.orcid0000-0002-0692-8155
dc.identifier.pmid36271980
dc.identifier.scopus2-s2.0-85140475485
dc.identifier.scopusqualityQ2
dc.identifier.startpage11997
dc.identifier.urihttps://doi.org/10.1007/s11033-022-08017-5
dc.identifier.urihttps://hdl.handle.net/20.500.12899/6068
dc.identifier.volume49
dc.identifier.wosWOS:000871174700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260612
dc.subjectEpileptogenesis
dc.subjectMir-342-3P
dc.subjectGrm2 Gene
dc.subjectHippocampus
dc.subjectPentylenetetrazole Kindling Model
dc.titleDownregulatory effect of miR-342-3p on epileptogenesis in the PTZ-kindling model
dc.typeArticle

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