Comparative Analysis of Antioxidant, Anticholinesterase, and Antibacterial Activity of Microbial Chondroitin Sulfate and Commercial Chondroitin Sulfate

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dc.authoridBoga, Mehmet/0000-0003-4163-9962|Unver, Tuba/0000-0002-8655-2716|Bingul, Murat/0000-0002-3909-0694
dc.contributor.authorUnver, Tuba
dc.contributor.authorErenler, Ayse Sebnem
dc.contributor.authorBingul, Murat
dc.contributor.authorBoga, Mehmet
dc.date.accessioned2025-10-24T18:08:46Z
dc.date.available2025-10-24T18:08:46Z
dc.date.issued2023
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractChondroitin synthesis was performed using the recombinant Escherichia coli(C2987) strain created by transforming the plasmid pETM6-PACF-vgb, which carries the genes responsible for chondroitin synthesis, kfoA, kfoC, kfoF, and the Vitreoscilla hemoglobin gene (vgb). Then, Microbial chondroitin sulfate (MCS)'s antioxidant, anticholinesterase, and antibacterial activity were compared with commercial chondroitin sulfate (CCS). The antioxidant studies revealed that the MCS and CCS samples could be potential targets for scavenging radicals and cupric ion reduction. MCS demonstrated better antioxidant properties in the ABTS assay with the IC50 value of 0.66 mg than CCS. MCS showed 2.5-fold for DPPH and almost 5-fold for ABTS(center dot)+ (with a value of 3.85 mg/mL) better activity than the CCS. However, the compounds were not active for cholinesterase enzyme inhibitions. In the antibacterial assay, the Minimum inhibitory concentration (MIC) values of MCS against S. aureus, E. aerogenes, E. coli, P. aeruginosa, and K. pneumoniae (0.12, 0.18, 0.12, 0.18, and 0.18 g/mL, respectively) were found to be greater than that of CCS (0.42, 0.48, 0.36, 0.36, and 0.36 g/mL, respectively). This study demonstrates that MCS is a potent pharmacological agent due to its physicochemical properties, and its usability as a therapeutic-preventive agent will shed light on future studies.
dc.identifier.doi10.1002/cbdv.202300924
dc.identifier.issn1612-1872
dc.identifier.issn1612-1880
dc.identifier.issue10
dc.identifier.pmid37615364
dc.identifier.urihttps://doi.org/10.1002/cbdv.202300924
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3278
dc.identifier.volume20
dc.identifier.wosWOS:001060373100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistry & Biodiversity
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectanimal-originated chondroitin sulfate; antibacterial activity; anticholinesterase activity; antioxidant agent; microbial chondroitin sulfate
dc.titleComparative Analysis of Antioxidant, Anticholinesterase, and Antibacterial Activity of Microbial Chondroitin Sulfate and Commercial Chondroitin Sulfate
dc.typeArticle

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