Silver(I)-NHC Complexes as Dual-Action Agents Against Pathogenic Acanthamoeba Trophozoites: Anti-Amoebic and Anti-Adhesion Activities

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dc.contributor.authorHkiri, Shaima
dc.contributor.authorSahin, Neslihan
dc.contributor.authorAkin-Polat, Zubeyda
dc.contributor.authorUstun, Elvan
dc.contributor.authorLy, Bui Minh Thu
dc.contributor.authorOzdemir, Ismail
dc.contributor.authorSemeril, David
dc.date.accessioned2026-06-19T06:37:44Z
dc.date.available2026-06-19T06:37:44Z
dc.date.issued2025
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractA series of six silver(I) complexes, namely bromo(1-benzyl-3-cinnamyl-benzimidazol-2-ylidene)silver (I) (1a), bromo[1-(4-methylbenzyl)-3-cinnamyl-benzimidazol-2-yliden]silver(I) (1b), bromo[1-(3-methoxylbenzyl)-3-cinnamyl-benzimidazol-2-yliden]silver(I) (1c), bromo[1-(3,5-dimethoxy-benzyl)-3-cinnamyl-benzimidazol-2-ylidene]silver(I) (1d), bromo[1-(naphthalen-1-ylmethyl)-3-cinnamyl-benzimidazol-2-ylidene]silver(I) (1e) and bromo[1-(pyren-1-ylmethyl)-3-cinnamyl-benzimidazol-2-yliden]silver(I) (1f), were synthetized and characterized by microanalyses and mass spectrometry and characterized by FT-IR and NMR spectroscopic techniques. The in vitro effects of silver(I) complexes on trophozoites of two Acanthamoeba isolates obtained from patients with keratitis were investigated. The parasites were exposed to concentrations of 10, 100 and 1000 mu M for 24, 48 and 72 h. The complexes exhibited potent, dose- and time-dependent activity. Complete inhibition was observed within 24 h at a concentration of 1000 mu M. At a concentration of 100 mu M, complexes 1c-e exhibited reduced viability to less than 10% within 48 to 72 h. At a concentration of 10 mu M, partial inhibition was observed. Preliminary morphological changes included the loss of acanthopodia, rounding, and detachment. These effects were not observed in the presence of the pre-ligands or commercially available silver compounds. Furthermore, molecular docking was utilized to analyze the molecules against Acanthamoeba castellanii CYP51, A. castellanii profilin IA, IB, and II. The highest recorded interactions were identified as -9.85 and -11.26 kcal/mol for 1e and 1f, respectively, when evaluated against the A. castellanii CYP51 structure.
dc.identifier.doi10.3390/ijms26199393
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.issue19
dc.identifier.orcid0000-0002-0587-7261
dc.identifier.orcid0000-0003-1498-4170
dc.identifier.orcid0000-0002-2582-4191
dc.identifier.pmid41096662
dc.identifier.scopus2-s2.0-105018893368
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/ijms26199393
dc.identifier.urihttps://hdl.handle.net/20.500.12899/5202
dc.identifier.volume26
dc.identifier.wosWOS:001593680600001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260612
dc.subjectSilver Complex
dc.subjectN-Heterocyclic Carbene
dc.subjectAcanthamoeba
dc.subjectIn Vitro Evaluation
dc.subjectAnti-Amoebic Activity
dc.subjectAnti-Adhesion Activity
dc.subjectMolecular Docking
dc.titleSilver(I)-NHC Complexes as Dual-Action Agents Against Pathogenic Acanthamoeba Trophozoites: Anti-Amoebic and Anti-Adhesion Activities
dc.typeArticle

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