Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis

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dc.authoridBoeckmann, Lars/0000-0003-0098-6546|Pachpor, Tejaswini/0000-0002-5449-5526|Jowhar, Ziad/0000-0002-3536-079X|Bloom, Kerry/0000-0002-3457-004X
dc.contributor.authorMishra, Prashant K.
dc.contributor.authorCiftci-Yilmaz, Sultan
dc.contributor.authorReynolds, David
dc.contributor.authorAu, Wei-Chun
dc.contributor.authorBoeckmann, Lars
dc.contributor.authorDittman, Lauren E.
dc.contributor.authorJowhar, Ziad
dc.date.accessioned2025-10-24T18:09:16Z
dc.date.available2025-10-24T18:09:16Z
dc.date.issued2016
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractSister chromatid cohesion is essential for tension-sensing mechanisms that monitor bipolar attachment of replicated chromatids in metaphase. Cohesion is mediated by the association of cohesins along the length of sister chromatid arms. In contrast, centromeric cohesin generates intrastrand cohesion and sister centromeres, while highly cohesin enriched, are separated by >800 nm at metaphase in yeast. Removal of cohesin is necessary for sister chromatid separation during anaphase, and this is regulated by evolutionarily conserved polo-like kinase (Cdc5 in yeast, Plk1 in humans). Here we address how high levels of cohesins at centromeric chromatin are removed. Cdc5 associates with centromeric chromatin and cohesin-associated regions. Maximum enrichment of Cdc5 in centromeric chromatin occurs during the metaphase-to-anaphase transition and coincides with the removal of chromosome- associated cohesin. Cdc5 interacts with cohesin in vivo, and cohesin is required for association of Cdc5 at centromeric chromatin. Cohesin removal from centromeric chromatin requires Cdc5 but removal at distal chromosomal arm sites does not. Our results define a novel role for Cdc5 in regulating removal of centromeric cohesins and faithful chromosome segregation.
dc.description.sponsorshipIntramural Research Program of the National Cancer Institute, National Institutes of Health; National Science Foundation; National Institutes of Health [R37 GM32238]; Cancer Research Society; Canadian Institutes of Health Research [MOP 82912]
dc.description.sponsorshipWe are highly thankful to Vincent Guacci and Robert Skibbens for strains, plasmids, and helpful suggestions. We thank Sue Biggins for plasmids and Jennifer Gerton for strains, Kathy McKinnon of the National Cancer Institute Vaccine Branch FACS Core for assistance with FACS and the Basrai laboratory for discussions. P.K.M., S.C.Y., W.C.A., L.B., L.E.D., Z.J., T.P., and M.A.B. were supported by the Intramural Research Program of the National Cancer Institute, National Institutes of Health; M.A.H. and D.R. by the National Science Foundation; E.Y. and K.B. by the National Institutes of Health (R37 GM32238); and D.D. by the Cancer Research Society and the Canadian Institutes of Health Research (MOP 82912).
dc.identifier.doi10.1091/mbc.E16-01-0004
dc.identifier.endpage2300
dc.identifier.issn1059-1524
dc.identifier.issn1939-4586
dc.identifier.issue14
dc.identifier.pmid27226485
dc.identifier.scopus2-s2.0-84978765545
dc.identifier.scopusqualityQ3
dc.identifier.startpage2286
dc.identifier.urihttps://doi.org/10.1091/mbc.E16-01-0004
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3558
dc.identifier.volume27
dc.identifier.wosWOS:000380823500012
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Soc Cell Biology
dc.relation.ispartofMolecular Biology Of The Cell
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20251023
dc.subjectSister-Chromatid Cohesion; Chromosome Transmission Fidelity; H3 Variant Cse4; Saccharomyces-Cerevisiae; Budding Yeast; Protein-Kinase; Mitotic Exit; Rec8 Phosphorylation; Anaphase Initiation; Somatic Mutations
dc.titlePolo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis
dc.typeArticle

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