Optimization of Phellinus hartigii extracts: Biological activities, and phenolic content analysis

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dc.contributor.authorUnal, Orhan
dc.contributor.authorGurgen, Aysenur
dc.contributor.authorKrupodorova, Tetiana
dc.contributor.authorSevindik, Mustafa
dc.contributor.authorKabaktepe, Sanli
dc.contributor.authorAkata, Ilgaz
dc.date.accessioned2026-06-19T06:38:16Z
dc.date.available2026-06-19T06:38:16Z
dc.date.issued2025
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractBackgroundMedicinal mushrooms are sources of natural substances with diverse biological functions. The study evaluated the biological activity of Phellinus hartigii (Allesch. & Schnabl) Pat. and optimized extraction conditions to the maximize its bioactive potential.MethodsExtraction was performed using a Soxhlet apparatus under varying conditions: temperatures (30, 50, and 70 degrees C), durations (1, 5.5, and 10 h), and ethanol/water ratios (0%, 50%, and 100%). Total antioxidant status (TAS) was analyzed across 17 experiments, and the optimal conditions were identified using response surface methodology (RSM). Extracts from optimal conditions were further analyzed for antioxidant capacity (Rel assay kits, DPPH, FRAP), anticholinesterase activity (acetyl- and butyrylcholinesterase inhibition), antiproliferative activity (A549 lung cancer cell line), total phenolic content (Folin-Ciocalteu method), and phenolic compound profile (LC-MS/MS).ResultsOptimal extraction conditions were determined to be 48.22 & ring;C, 9.04 h, and an ethanol/water ratio of 52.22%. The extract exhibited significant antiproliferative effects against the A549 lung cancer cells, with activity increasing in a concentration-dependent manner. The inhibition values (IC50) of acetylcholinesterase and butyrylcholinesterase were 21.29 +/- 0.41 and 35.51 +/- 0.53 mu g/mL, respectively. The TPC (total phenolic content) value of the optimized extract was determined as 88.21 +/- 1.50 mg/g, FRAP value as 137.81 +/- 1.72 mg/g, DPPH value as 106.07 +/- 2.44 mg/g, TOS (total oxidant status) value as 9.27 +/- 0.06 mu mol/L, TAS value as 4.98 +/- 0.03 mmol/L and OSI (oxidative stress index) value as 0.19 +/- 0.002. LC-MS/MS analysis identified nine phenolic compounds, with gallic acid and catechin hydrate as the most abundant.ConclusionsThe extract of P. hartigii obtained under optimal conditions demonstrated substantial antioxidant, anticholinesterase, and antiproliferative activities, highlighting its therapeutic potential.
dc.identifier.doi10.1186/s12906-025-04851-9
dc.identifier.issn2662-7671
dc.identifier.issue1
dc.identifier.orcid0000-0001-8286-9225
dc.identifier.pmid40133842
dc.identifier.scopus2-s2.0-105000951885
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/s12906-025-04851-9
dc.identifier.urihttps://hdl.handle.net/20.500.12899/5479
dc.identifier.volume25
dc.identifier.wosWOS:001451500700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBmc
dc.relation.ispartofBmc Complementary Medicine and Therapies
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260612
dc.subjectAntialzheimer
dc.subjectAnticancer
dc.subjectAntioxidant
dc.subjectPhellinus Hartigii
dc.subjectPhenolic
dc.subjectResponse Surface Method
dc.titleOptimization of Phellinus hartigii extracts: Biological activities, and phenolic content analysis
dc.typeArticle

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