The Cytotoxicity, DNA Fragmentation, and Decreasing Velocity Induced By Chromium(III) Oxide on Rainbow Trout Spermatozoa

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dc.authorid0000-0002-2966-9627en_US
dc.contributor.authorÖzgür, Mustafa Erkan
dc.contributor.authorUlu, Ahmet
dc.contributor.authorGürses, Canbolat
dc.contributor.authorÖzcan, İmren
dc.contributor.authorSamir Abbas Ali, Noma
dc.contributor.authorKöytepe, Süleyman
dc.contributor.authorAteş, Burhan
dc.date.accessioned2022-04-06T10:27:40Z
dc.date.available2022-04-06T10:27:40Z
dc.date.issued2022en_US
dc.departmentMTÖ Üniversitesi, Doğanşehir Vahap Küçük Meslek Yüksekokulu, Su Ürünleri Bölümüen_US
dc.description.abstractThe present study aimed to determine the cytotoxicity of chromium(III) oxide micro particles (Cr2O3-Ps) in rainbow trout (Oncorhynchus mykiss) spermatozoa. Firstly, Cr2O3-Ps were synthesized and structurally characterized the surface, morphological for particle size and thermal properties. In addition, its structural and elemental purity was determined using energy-dispersive X-ray (EDX) spectrum and elemental maps. Structural purity, thermal properties, and stability of Cr2O3-Ps were also examined in detail by performing thermal analysis techniques. The cytotoxicity of Cr2O3-Ps was measured by the observation of velocities, antioxidant activities, and DNA damages in rainbow trout spermatozoa after exposure during 3 h in vitro incubation. The straight line velocity (VSL), the curvilinear velocity (VCL), and the angular path velocity (VAP) of spermatozoa decreased after exposure to Cr2O3-Ps. While the superoxide dismutase (SOD) and the catalase (CAT) decreased, the lipid peroxidation increased in a dose-dependent manner. However, the total glutathione (tGSH) was not affected in this period. DNA damages were also determined in spermatozoa using Comet assay. According to DNA in tail (%) data, DNA damages have been detected with gradually increasing concentrations of Cr2O3-Ps. Furthermore, all of class types which are categorized as the intensity of DNA fragmentation has been observed between 50 and 500 µg/L concentrations of Cr2O3-Ps exposed to rainbow trout spermatozoa. At the end of this study, we determined that the effective concentrations (EC50) were 76.67 µg/L for VSL and 87.77 µg/L for VCL. Finally, these results about Cr2O3-Ps may say to be major risk concentrations over 70 µg/L for fish reproduction in aquatic environments.en_US
dc.identifier.citationÖzgür, M. E., Ulu, A., Gürses, C., Özcan, İ., Noma, S. A. A., Köytepe, S., & Ateş, B. (2022). The Cytotoxicity, DNA Fragmentation, and Decreasing Velocity Induced by Chromium (III) Oxide on Rainbow Trout Spermatozoa.Biological Trace Element Research(1-16).en_US
dc.identifier.doi10.1007/s12011-022-03211-9
dc.identifier.endpage16en_US
dc.identifier.pmid35368229
dc.identifier.scopus2-s2.0-85127553178en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1007/s12011-022-03211-9
dc.identifier.urihttps://hdl.handle.net/20.500.12899/919
dc.identifier.wosWOS:000777529900002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖzgür, Mustafa Erkan
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiological Trace Element Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCr2O3en_US
dc.subjectCytotoxicityen_US
dc.subjectDNA damagesen_US
dc.subjectOncorhynchus mykissen_US
dc.subjectSpermatozoaen_US
dc.titleThe Cytotoxicity, DNA Fragmentation, and Decreasing Velocity Induced By Chromium(III) Oxide on Rainbow Trout Spermatozoaen_US
dc.typeArticleen_US

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